Interaction of difluoro-oxaloacetate with aspartate transaminase

Dffluoro-oxaloacetate with Aspartate Transaminase

Difluoro-oxaloacetate interacts with the aldimine form of aspartate transaminase to give a complex, the dissociation constant ofwhich has been determined spectrophotometrically and by 19F n.m.r. (nuclear magnetic resonance). The 19F n.m.r. line-width-pH and chemical-shift-pH profiles of difluoro-oxaloacetate in the presence of the aldimine form of the enzyme both show inflexion points in the pH...

Interaction between beta-fluoro-oxaloacetate and glutamate-aspartate transaminase of heart mitochondria.

Interaction between p-Fluoro-oxaloacetate and Glutamate- Aspartate Transaminase of Heart Mitochondria”

In a previous publication (1) we have described the synthesis and chemical properties of /3-fluoro-oxaloacetic acid. This acid was found to be a potent inhibitor of mitochondriil malic dehydrogenase (1,2). It is probable that fluoro-oxaloacetate inhibits other enzymes which react with oxaloacetate. We have performed further experiments in order to test this hypothesis. As a part of this study t...

Urinary aspartate transaminase in childhood.

The interaction of cycloserine with glutamate aspartate transaminase as measured by fluorescence spectroscopy.

The reaction of cycloserine with pyridoxal5-phosphate has been investigated by fluorescence spectroscopy. The substantial change in fluorescence intensity at 450 rnp that follows the reaction of cycloserine with pyridoxal5-phosphate was used to monitor the release of the cofactor from the active site of the enzyme glutamate aspartate transaminase. A kinetic analysis of the fluorescence measurem...